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author:"Lee HJ"
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3D View
Consensus map of Cx43/GJA1 gap junction channel in the presence of diC8-PIP2 (8-fold molar excess)
Release date: June 11, 2025
Resolution: 3.1 Å
EM Method: Single-particle

Lee HJ, Cha HJ, Woo JS

To Be Published

Sample:
  • Dodecameric complex of human cx43-m257 in lipid nanodiscs (pope) in the presence of dic8-pip2 (7.5-fold molar excess) (Cellular component from Homo sapiens)
  • A c-terminal truncated mutant of gap junction alpha-1 protein (cx43-m257) (Protein from Homo sapiens)
3D View
Consensus map of Cx43/GJA1 gap junction channel in the presence of diC8-PIP2 (16-fold molar excess)
Release date: June 11, 2025
Resolution: 3.0 Å
EM Method: Single-particle

Lee HJ, Cha HJ, Woo JS

To Be Published

Sample:
  • Dodecameric complex of human cx43-m257 in lipid nanodiscs (pope) in the presence of dic8-pip2 (16-fold molar excess) (Cellular component from Homo sapiens)
  • A c-terminal truncated mutant of gap junction alpha-1 protein (cx43-m257) (Protein from Homo sapiens)
3D View
Structure of Cx43/GJA1 gap junction intercellular channel in complex with diC8-PIP2
Release date: June 11, 2025
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8xbm
Q-score: 0.462

Lee HJ, Cha HJ, Woo JS

To Be Published

Sample:
  • Dodecameric complex of human cx43-m257 in lipid nanodiscs (pope) in the presence of dic8-pip2 (16-fold molar excess) (Cellular component from Homo sapiens)
  • Gap junction alpha-1 protein (29 kDa, Protein from Homo sapiens)
  • [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
3D View
Cryo-EM structure of the mouse TRPM3 alpha 2 channel in complex with primidone
Release date: Jan. 22, 2025
Resolution: 3.24 Å
EM Method: Single-particle
Fitted PDBs: 9b28
Q-score: 0.438

Yin Y, Park CG, Feng S, Guan Z, Lee HJ, Zhang F, Sharma K, Borgnia MJ, Im W, Lee SY

Nat Struct Mol Biol (2025) 32 pp. 828-840 [ DOI: doi:10.1038/s41594-024-01463-8 Pubmed: 39809942 ]

Sample:
  • Cholesterol hemisuccinate (486 Da, Ligand)
  • [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate (717 Da, Ligand)
  • Primidone (218 Da, Ligand)
  • Transient receptor potential cation channel, subfamily m, member 3 (198 kDa, Protein from Mus musculus)
  • Transient receptor potential cation channel subfamily m member 3 (Complex from Mus musculus)
[Show more]
3D View
Cryo-EM structure of the mouse TRPM3 alpha 2 channel in complex with cholesteryl hemisuccinate
Release date: Jan. 22, 2025
Resolution: 3.13 Å
EM Method: Single-particle
Fitted PDBs: 9b29
Q-score: 0.394

Yin Y, Park CG, Feng S, Guan Z, Lee HJ, Zhang F, Sharma K, Borgnia MJ, Im W, Lee SY

Nat Struct Mol Biol (2025) 32 pp. 828-840 [ DOI: doi:10.1038/s41594-024-01463-8 Pubmed: 39809942 ]

Sample:
  • Cholesterol hemisuccinate (486 Da, Ligand)
  • [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate (717 Da, Ligand)
  • 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (760 Da, Ligand)
  • Transient receptor potential cation channel, subfamily m, member 3 (198 kDa, Protein from Mus musculus)
  • 1,2-dilauroyl-sn-glycero-3-phosphate (535 Da, Ligand)
  • Transient receptor potential cation channel subfamily m member 3 (Complex from Mus musculus)
[Show more]
3D View
Cryo-EM structure of the mouse TRPM3 alpha 2 channel in complex with the neurosteroid pregnenolone sulfate and the synthetic agonist CIM 0216
Release date: Jan. 22, 2025
Resolution: 3.82 Å
EM Method: Single-particle
Fitted PDBs: 9b2a
Q-score: 0.34

Yin Y, Park CG, Feng S, Guan Z, Lee HJ, Zhang F, Sharma K, Borgnia MJ, Im W, Lee SY

Nat Struct Mol Biol (2025) 32 pp. 828-840 [ DOI: doi:10.1038/s41594-024-01463-8 Pubmed: 39809942 ]

Sample:
  • [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate (717 Da, Ligand)
  • (2s)-2-(3,4-dihydroquinolin-1(2h)-yl)-n-(5-methyl-1,2-oxazol-3-yl)-2-phenylacetamide (347 Da, Ligand)
  • Pregnenolone sulfate (396 Da, Ligand)
  • Transient receptor potential cation channel, subfamily m, member 3 (198 kDa, Protein from Mus musculus)
  • Transient receptor potential cation channel subfamily m member 3 (Complex from Mus musculus)
[Show more]
3D View
Human Cx36/GJD2 (N-terminal deletion mutant) gap junction channel in soybean lipids (D6 symmetry)
Release date: Aug. 30, 2023
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7xl8
Q-score: 0.532

Lee SN, Cho HJ, Jeong H, Ryu B, Lee HJ, Kim M, Yoo J, Woo JS, Lee HH

Nat Commun (2023) 14 pp. 1347-1347 [ DOI: doi:10.1038/s41467-023-37040-8 Pubmed: 36906653 ]

Sample:
  • 1,2-dimyristoyl-rac-glycero-3-phosphocholine (677 Da, Ligand)
  • Gap junction delta-2 protein (36 kDa, Protein from Homo sapiens)
  • Cx36-deln8 (Complex from Homo sapiens)
3D View
Human Cx36/GJD2 (N-terminal deletion mutant) gap junction channel in soybean lipids (C1 symmetry)
Release date: Aug. 9, 2023
Resolution: 3.41 Å
EM Method: Single-particle

Lee SN, Cho HJ, Jeong H, Ryu B, Lee HJ, Kim M, Yoo J, Woo JS, Lee HH

Nat Commun (2023) 14 pp. 1347-1347 [ DOI: doi:10.1038/s41467-023-37040-8 Pubmed: 36906653 ]

Sample:
  • Cx36-deln8 (Complex from Homo sapiens)
3D View
Human Cx36/GJD2 gap junction channel with pore-lining N-terminal helices in the presence of Magnesium
Release date: May 3, 2023
Resolution: 2.68 Å
EM Method: Single-particle

Lee SN, Cho HJ, Jeong H, Ryu B, Lee HJ, Lee HH, Woo JS

To Be Published

Sample:
  • Cx36 (Complex from Homo sapiens)
3D View
Human Cx36/GJD2 gap junction channel in detergents
Release date: April 26, 2023
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7xkk
Q-score: 0.545

Lee SN, Cho HJ, Jeong H, Ryu B, Lee HJ, Kim M, Yoo J, Woo JS, Lee HH

Nat Commun (2023) 14 pp. 1347-1347 [ DOI: doi:10.1038/s41467-023-37040-8 Pubmed: 36906653 ]

Sample:
  • Gap junction delta-2 protein (37 kDa, Protein from Homo sapiens)
  • Cx36 (Complex from Homo sapiens)
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