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› 1znb

X-ray diffraction
1.85Å resolution

METALLO-BETA-LACTAMASE

Released:
DOI: 10.2210/pdb1znb/pdb
PDB entry 1znb coloured by chain, front view.
PDB entry 1znb coloured by chain, side view.
PDB entry 1znb coloured by chain, top view.
Source organism: Bacteroides fragilis
Primary publication:
Crystal structure of the wide-spectrum binuclear zinc beta-lactamase from Bacteroides fragilis.
Concha NO, Rasmussen BA, Bush K, Herzberg O
Structure 4 823-36 (1996)
PMID: 8805566

Function and Biology Details

Reaction catalysed: 
a beta-lactam + H2O = a substituted beta-amino acid.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150609 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase type 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 232 amino acids
Theoretical weight: 25.27 KDa
Source organism: Bacteroides fragilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P25910 (Residues: 18-249; Coverage: 100%)
Gene names: ccrA, cfiA
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

2 bound ligands:
Ligand ZN 4 x ZN
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU, NSLS BEAMLINE X12C
Spacegroup: P43212
Unit cell:
a: 78.298Å b: 78.298Å c: 140.916Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.167 0.241
Expression system: Escherichia coli BL21

Quick links

Citations

28 review citations

Carbapenemases: the versatile beta-lactamases.
Queenan et al. (2007)
27 more

11 mentions without citation

Metallo-β-lactamase structure and function.
Palzkill T. (2013)
10 more