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› 6qc4

Electron Microscopy
4.6Å resolution

Ovine respiratory supercomplex I+III2 open class 3

Released:
DOI: 10.2210/pdb6qc4/pdb
PDB entry 6qc4 coloured by chain, front view.
PDB entry 6qc4 coloured by chain, side view.
PDB entry 6qc4 coloured by chain, top view.
Source organism: Ovis aries
Primary publication:
Structures of Respiratory Supercomplex I+III2 Reveal Functional and Conformational Crosstalk.
Letts JA, Fiedorczuk K, Degliesposti G, Skehel M, Sazanov LA
OpenAccess logo Mol Cell 75 1131-1146.e6 (2019)
PMID: 31492636
Related structures: EMD-4496

Function and Biology Details

Reaction catalysed: 
a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 65-mer (preferred)
PDBe Complex ID:
PDB-CPX-131279 (preferred)
Entry contents:
54 distinct polypeptide molecules
Macromolecules (54 distinct):
UQCRC1 Chains: a1, a3
Molecule details ›
Chains: a1, a3
Length: 446 amino acids
Theoretical weight: 49.39 KDa
Source organism: Ovis aries
Ubiquinol-cytochrome c reductase core protein 2 Chains: a2, a4
Molecule details ›
Chains: a2, a4
Length: 439 amino acids
Theoretical weight: 46.66 KDa
Source organism: Ovis aries
Cytochrome b Chains: b1, b2
Molecule details ›
Chains: b1, b2
Length: 379 amino acids
Theoretical weight: 42.88 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: P24959 (Residues: 1-379; Coverage: 100%)
Gene names: COB, CYTB, MT-CYB, MTCYB
Sequence domains:
Cytochrome c1 Chains: c1, c2
Molecule details ›
Chains: c1, c2
Length: 240 amino acids
Theoretical weight: 27.32 KDa
Source organism: Ovis aries
Cytochrome b-c1 complex subunit Rieske, mitochondrial Chains: f1, f2
Molecule details ›
Chains: f1, f2
Length: 196 amino acids
Theoretical weight: 21.65 KDa
Source organism: Ovis aries
UQCRB Chains: d1, d2
Molecule details ›
Chains: d1, d2
Length: 110 amino acids
Theoretical weight: 13.43 KDa
Source organism: Ovis aries
Cytochrome b-c1 complex subunit 8 Chains: q1, q2
Molecule details ›
Chains: q1, q2
Length: 81 amino acids
Theoretical weight: 9.61 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PUP9 (Residues: 2-82; Coverage: 99%)
Gene name: LOC101121420
Sequence domains: UcrQ family
Cytochrome b-c1 complex subunit 6 Chains: h1, h2
Molecule details ›
Chains: h1, h2
Length: 78 amino acids
Theoretical weight: 9.22 KDa
Source organism: Ovis aries
UQCRFS1N Chains: x1, x2
Molecule details ›
Chains: x1, x2
Length: 78 amino acids
Theoretical weight: 6.66 KDa
Source organism: Ovis aries
Ubiquinol-cytochrome c reductase, complex III subunit X Chains: i1, i2
Molecule details ›
Chains: i1, i2
Length: 63 amino acids
Theoretical weight: 7.31 KDa
Source organism: Ovis aries
NADH-ubiquinone oxidoreductase chain 3 Chain: D3
Molecule details ›
Chain: D3
Length: 115 amino acids
Theoretical weight: 13.11 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: O78753 (Residues: 1-115; Coverage: 100%)
Gene names: MT-ND3, MTND3, NADH3, ND3
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase, chain 3
NADH-ubiquinone oxidoreductase chain 1 Chain: D1
Molecule details ›
Chain: D1
Length: 318 amino acids
Theoretical weight: 35.88 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: O78747 (Residues: 1-318; Coverage: 100%)
Gene names: MT-ND1, MTND1, NADH1, ND1
Sequence domains: NADH dehydrogenase
NADH-ubiquinone oxidoreductase chain 6 Chain: D6
Molecule details ›
Chain: D6
Length: 175 amino acids
Theoretical weight: 19.13 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: O78757 (Residues: 1-175; Coverage: 100%)
Gene names: MT-ND6, MTND6, NADH6, ND6
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase chain 6
NADH-ubiquinone oxidoreductase chain 4L Chain: 4L
Molecule details ›
Chain: 4L
Length: 98 amino acids
Theoretical weight: 10.84 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: O78754 (Residues: 1-98; Coverage: 100%)
Gene names: MT-ND4L, MTND4L, NADH4L, ND4L
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase chain 4L
NADH-ubiquinone oxidoreductase chain 5 Chain: D5
Molecule details ›
Chain: D5
Length: 606 amino acids
Theoretical weight: 68.41 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: O78756 (Residues: 1-606; Coverage: 100%)
Gene names: MT-ND5, MTND5, NADH5, ND5
Sequence domains:
NADH-ubiquinone oxidoreductase chain 4 Chain: D4
Molecule details ›
Chain: D4
Length: 459 amino acids
Theoretical weight: 52.06 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: O78755 (Residues: 1-459; Coverage: 100%)
Gene names: MT-ND4, MTND4, NADH4, ND4
Sequence domains:
NADH-ubiquinone oxidoreductase chain 2 Chain: D2
Molecule details ›
Chain: D2
Length: 347 amino acids
Theoretical weight: 39.15 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: O78748 (Residues: 1-347; Coverage: 100%)
Gene names: MT-ND2, MTND2, NADH2, ND2
Sequence domains:
NDUFA11 Chain: AK
Molecule details ›
Chain: AK
Length: 140 amino acids
Theoretical weight: 14.61 KDa
Source organism: Ovis aries
NADH:ubiquinone oxidoreductase subunit B5 Chain: B5
Molecule details ›
Chain: B5
Length: 143 amino acids
Theoretical weight: 16.71 KDa
Source organism: Ovis aries
Acyl carrier protein Chains: AA, AB
Molecule details ›
Chains: AA, AB
Length: 88 amino acids
Theoretical weight: 10.12 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5NQT7 (Residues: 70-157; Coverage: 56%)
Sequence domains: Phosphopantetheine attachment site
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 Chain: A8
Molecule details ›
Chain: A8
Length: 171 amino acids
Theoretical weight: 20.01 KDa
Source organism: Ovis aries
NDUFB10 Chain: BJ
Molecule details ›
Chain: BJ
Length: 175 amino acids
Theoretical weight: 20.88 KDa
Source organism: Ovis aries
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial Chain: AJ
Molecule details ›
Chain: AJ
Length: 320 amino acids
Theoretical weight: 36.8 KDa
Source organism: Ovis aries
NADH:ubiquinone oxidoreductase subunit S5 Chain: S5
Molecule details ›
Chain: S5
Length: 105 amino acids
Theoretical weight: 12.47 KDa
Source organism: Ovis aries
NADH:ubiquinone oxidoreductase subunit A3 Chain: A3
Molecule details ›
Chain: A3
Length: 83 amino acids
Theoretical weight: 9.19 KDa
Source organism: Ovis aries
NADH:ubiquinone oxidoreductase subunit B3 Chain: B3
Molecule details ›
Chain: B3
Length: 97 amino acids
Theoretical weight: 11.02 KDa
Source organism: Ovis aries
NADH dehydrogenase [ubiquinone] 1 subunit C2 Chain: C2
Molecule details ›
Chain: C2
Length: 120 amino acids
Theoretical weight: 14.23 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5P9Q8 (Residues: 3-122; Coverage: 98%)
Gene name: NDUFC2
Sequence domains: NADH-ubiquinone oxidoreductase subunit b14.5b (NDUFC2)
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 Chain: B4
Molecule details ›
Chain: B4
Length: 128 amino acids
Theoretical weight: 14.98 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5QF73 (Residues: 3-130; Coverage: 99%)
Gene name: NDUFB4
Sequence domains: NADH-ubiquinone oxidoreductase B15 subunit (NDUFB4)
NDUFA13 Chain: AM
Molecule details ›
Chain: AM
Length: 143 amino acids
Theoretical weight: 16.64 KDa
Source organism: Ovis aries
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 Chain: B6
Molecule details ›
Chain: B6
Length: 127 amino acids
Theoretical weight: 15.43 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PZE3 (Residues: 2-128; Coverage: 99%)
Gene name: NDUFB6
Sequence domains: NADH:ubiquinone oxidoreductase, NDUFB6/B17 subunit
NADH:ubiquinone oxidoreductase subunit B7 Chain: B7
Molecule details ›
Chain: B7
Length: 119 amino acids
Theoretical weight: 14.65 KDa
Source organism: Ovis aries
NADH:ubiquinone oxidoreductase subunit B9 Chain: B9
Molecule details ›
Chain: B9
Length: 178 amino acids
Theoretical weight: 21.65 KDa
Source organism: Ovis aries
NADH:ubiquinone oxidoreductase subunit B2 Chain: B2
Molecule details ›
Chain: B2
Length: 72 amino acids
Theoretical weight: 8.5 KDa
Source organism: Ovis aries
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial Chain: B8
Molecule details ›
Chain: B8
Length: 158 amino acids
Theoretical weight: 18.82 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5Q1B0 (Residues: 29-186; Coverage: 85%)
Gene name: NDUFB8
Sequence domains: NADH-ubiquinone oxidoreductase ASHI subunit (CI-ASHI or NDUFB8)
NDUFB11 Chain: BK
Molecule details ›
Chain: BK
Length: 125 amino acids
Theoretical weight: 14.46 KDa
Source organism: Ovis aries
NDUFC1 Chain: C1
Molecule details ›
Chain: C1
Length: 49 amino acids
Theoretical weight: 5.81 KDa
Source organism: Ovis aries
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 Chain: B1
Molecule details ›
Chain: B1
Length: 57 amino acids
Theoretical weight: 6.93 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5QG39 (Residues: 2-58; Coverage: 98%)
Gene name: LOC114115567
Sequence domains: MNLL subunit
NDUFA1 Chain: A1
Molecule details ›
Chain: A1
Length: 70 amino acids
Theoretical weight: 8.21 KDa
Source organism: Ovis aries
NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial Chain: V1
Molecule details ›
Chain: V1
Length: 445 amino acids
Theoretical weight: 48.68 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PUX0 (Residues: 20-464; Coverage: 96%)
Gene name: NDUFV1
Sequence domains:
NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial Chain: V2
Molecule details ›
Chain: V2
Length: 217 amino acids
Theoretical weight: 23.84 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5NRY1 (Residues: 30-246; Coverage: 88%)
Gene name: NDUFV2
Sequence domains: Thioredoxin-like [2Fe-2S] ferredoxin
NADH:ubiquinone oxidoreductase core subunit S1 Chain: S1
Molecule details ›
Chain: S1
Length: 704 amino acids
Theoretical weight: 77.03 KDa
Source organism: Ovis aries
NDUFS2 Chain: S2
Molecule details ›
Chain: S2
Length: 430 amino acids
Theoretical weight: 49.19 KDa
Source organism: Ovis aries
NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial Chain: S3
Molecule details ›
Chain: S3
Length: 228 amino acids
Theoretical weight: 26.44 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PB27 (Residues: 39-266; Coverage: 86%)
Gene name: NDUFS3
Sequence domains: Respiratory-chain NADH dehydrogenase, 30 Kd subunit
NDUFS7 Chain: S7
Molecule details ›
Chain: S7
Length: 179 amino acids
Theoretical weight: 20.1 KDa
Source organism: Ovis aries
NDUFS8 Chain: S8
Molecule details ›
Chain: S8
Length: 176 amino acids
Theoretical weight: 20.22 KDa
Source organism: Ovis aries
NDUFV3 Chain: V3
Molecule details ›
Chain: V3
Length: 75 amino acids
Theoretical weight: 8.42 KDa
Source organism: Ovis aries
NDUFS6 Chain: S6
Molecule details ›
Chain: S6
Length: 96 amino acids
Theoretical weight: 10.65 KDa
Source organism: Ovis aries
NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial Chain: S4
Molecule details ›
Chain: S4
Length: 133 amino acids
Theoretical weight: 15.38 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PE07 (Residues: 38-170; Coverage: 78%)
Gene name: NDUFS4
Sequence domains: NADH dehydrogenase ubiquinone Fe-S protein 4
NADH:ubiquinone oxidoreductase subunit A9 Chain: A9
Molecule details ›
Chain: A9
Length: 338 amino acids
Theoretical weight: 38.66 KDa
Source organism: Ovis aries
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 Chain: A2
Molecule details ›
Chain: A2
Length: 98 amino acids
Theoretical weight: 10.97 KDa
Source organism: Ovis aries
NDUFA5 Chain: A5
Molecule details ›
Chain: A5
Length: 115 amino acids
Theoretical weight: 13.16 KDa
Source organism: Ovis aries
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 Chain: A6
Molecule details ›
Chain: A6
Length: 127 amino acids
Theoretical weight: 14.92 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5QC06 (Residues: 14-140; Coverage: 91%)
Gene name: NDUFA6
Sequence domains: Complex 1 protein (LYR family)
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 Chain: A7
Molecule details ›
Chain: A7
Length: 112 amino acids
Theoretical weight: 12.4 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5P0I2 (Residues: 3-114; Coverage: 98%)
Gene name: NDUFA7
Sequence domains: NADH:ubiquinone oxidoreductase subunit B14.5a (Complex I-B14.5a)
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 Chain: AL
Molecule details ›
Chain: AL
Length: 145 amino acids
Theoretical weight: 17.12 KDa
Source organism: Ovis aries

Ligands and Environments

9 bound ligands:
Ligand HEM 4 x HEM
Ligand HEC 2 x HEC
Ligand FES 4 x FES
Ligand PC1 1 x PC1
Ligand ZMP 2 x ZMP
Ligand SF4 6 x SF4
Ligand FMN 1 x FMN
Ligand ZN 1 x ZN
Ligand NDP 1 x NDP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.6Å
Relevant EMDB volumes: EMD-4496

Quick links

Citations

24 review citations

Mitochondrial Metabolism as a Target for Cancer Therapy.
Vasan et al. (2020)
23 more

2 mentions without citation

Structures of Respiratory Supercomplex I+III2 Reveal Functional and Conformational Crosstalk.
Letts et al. (2019)
1 more